experiment h1299 cellswere transfectedwithm50super 8×top flash (Addgene inc)

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Addgene inc experiment h1299 cellswere transfectedwithm50super 8×top flash
Experiment H1299 Cellswere Transfectedwithm50super 8×Top Flash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 573 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m50+super+8x+top+flash/M50+Super+8x+TOPFlash+(Plasmid+%2312456)/pm40721857-300-2-6
Average 96 stars, based on 573 article reviews

experiment h1299 cellswere transfectedwithm50super 8×top flash - by Bioz Stars, 2026-09

96/100 stars

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Transfection:

Article Title: Blocking the functional domain of cancer cell surface TIP1 upregulates Midkine via the β-catenin/Wnt signaling pathway.
Article Snippet: Drug resistance exhibited by cancer cells remains one of the primary reasons for the failure of therapeutic approaches to increase the survival of cancer patients.. Marginal improvement in therapeutic efficacy with current treatment approaches for non-small cell lung cancer (NSCLC) mandates new treatment strategies.. Tax interacting Protein-1 (TIP1) is a radiation-inducible molecular target involved in various cancer pathways.

Article Title: NCAM and attached polysialic acid affect behaviors of breast epithelial cells through differential signaling pathways
Article Snippet: .. Cells were seeded onto 24-well plates and transfected using Lipofectamine 2000 (Invitrogen) with 1 μg M50 Super 8X TOP Flash (Plasmid 12456) or M51 Super 8X FOP Flash (Plasmid 12457) reporter vector (Addgene), together with 0.05 μg internal pRL-TK Renilla plasmid (Promega). .. Cells were processed 48 h after co-transfection for luciferase reporter activity using a Dual Luciferase Reporter System (Promega).

Article Title: NCAM and attached polysialic acid affect behaviors of breast epithelial cells through differential signaling pathways.
Article Snippet: .. Cells were seeded into 24-well plates and transfected using Lipofectamine 2000 (Invitrogen) with 1 μg M50 Super 8x TOP Flash (Plasmid 12456) or M51 Super 8x FOP Flash (Plasmid 12457) reporter vector (Addgene, Cambridge, USA), together with 0.05 μg internal pRL-TK Renilla plasmid (Promega, Madison, USA). .. Cells were processed 48 h after co-transfection for luciferase reporter activity using a Dual Luciferase Reporter System (Promega).

Article Title: NCAM and attached polysialic acid affect behaviors of breast epithelial cells through differential signaling pathways
Article Snippet: .. Cells were seeded into 24-well plates and transfected using Lipofectamine 2000 (Invitrogen) with 1 μg M50 Super 8x TOP Flash (Plasmid 12456) or M51 Super 8x FOP Flash (Plasmid 12457) reporter vector (Addgene, Cambridge, USA), together with 0.05 μg internal pRL-TK Renilla plasmid (Promega, Madison, USA). .. Cells were processed 48 h after co-transfection for luciferase reporter activity using a Dual Luciferase Reporter System (Promega).

Article Title: PBRM1 regulates the stress response in epithelial cells
Article Snippet: Confocal laser scanning microscopy experiments were conducted using the Zeiss LSM 880 Upright Confocal. .. NMuMG cells were transfected with 10:1 ratio of M50 Super 8x Top flash (Addgene 12456) to pcDNA3.1.CMV-renilla-Neo. ..

Control:

Article Title: ZBTB20 regulates WNT/CTNNB1 signalling pathway by suppressing PPARG during hepatocellular carcinoma tumourigenesis
Article Snippet: After 24 hours post-transfection, luciferase activity was measured by a dual-luciferase reporter assay according to the manufacturer's instructions using the Glomax 20/20 Luminometer (Promega). .. TOP/FOP-Flash luciferase reporter assay The CTNNB1 reporter plasmids, consisting of M50 Super 8x TOP-Flash (Addgene plasmid #12456; http://n2t.net/addgene:12456; RRID:Addgene_12456) and its mutant control, M51 Super 8x FOP-Flash (Addgene plasmid #12457; http://n2t.net/addgene:12457; RRID:Addgene_12457) were gifts from Dr Randall Moon [11]. .. The TOP/FOP-Flash reporters, pRL-TK (Promega) and pPB/SB-Flag-ZBTB20 were co-transfected into PLC/PRF/5 and Hep3B cells, at a density of 6 X 104 cells onto 12-well plates.

Binding Assay:

Article Title: Tryptophan-kynurenine pathway attenuates β-catenin-dependent pro-parasitic role of STING-TICAM2-IRF3-IDO1 signalosome in Toxoplasma gondii infection
Article Snippet: .. M50 Super 8x Top-Flash (Top-Flash, addgene-12456) where seven TCF/LEF binding sites are present upstream of firefly luciferase reporter, and M51 Super 8x Fop-Flash (Top-Flash mutant, addgene-12457), where mutated TCF/LEF binding sites are present upstream of luciferase reporter , were used for luciferase reporter assay. ..

Luciferase:

Mutagenesis:

Reporter Assay:

Plasmid Preparation:

Article Title: NCAM and attached polysialic acid affect behaviors of breast epithelial cells through differential signaling pathways
Article Snippet: .. Cells were seeded into 24-well plates and transfected using Lipofectamine 2000 (Invitrogen) with 1 μg M50 Super 8x TOP Flash (Plasmid 12456) or M51 Super 8x FOP Flash (Plasmid 12457) reporter vector (Addgene, Cambridge, USA), together with 0.05 μg internal pRL-TK Renilla plasmid (Promega, Madison, USA). .. Cells were processed 48 h after co-transfection for luciferase reporter activity using a Dual Luciferase Reporter System (Promega).

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